The gene encoding a hybrid ubiquitin fusion protein involved in ribosome biogenesis is essential for growth

• DOI: 10.1016/s1567-1356(01)00056-3
• Publication Date: 2002-10-14T15:56:21Z
• Authors: P ROIG; D GOZALBO
• Keywords: ribosome biogenesis

We have constructed a conditional null mutant Candida albicans strain for the UBI3 gene which encodes ubiquitin fusion protein involved in ribosome biogenesis. A one-step disruption procedure, using plasmid pCaDis, was designed to place second copy of under control tightly regulated MET3 promoter C. heterozygous (UBI3/Δubi3::hisG), previously isolated first step ura-blaster protocol. Analysis repressing and inducing conditions indicates that is an essential whose expression required growth albicans.