Properties of the -derived constitutive promoter, assessed using an HSA reporter gene

• DOI: 10.1016/s1567-1356(03)00150-8
• Publication Date: 2003-06-09T19:53:54Z
• Authors: J HEO; W HONG; E CHO; M KIM; J KIM; C KIM; S RHEE; H KANG
• Keywords: Human serum albumin

The glyceraldehyde-3-phosphate dehydrogenase promoter, P(GAP), was employed to direct the constitutive expression of recombinant human serum albumin (HSA) in Hansenula polymorpha. A set integration vectors containing HSA cDNA under control P(GAP) constructed and elemental parameters affecting from were analyzed. presence a 5'-untranslated region derived vector into GAP locus shown improve P(GAP). Glycerol supported higher level along with cell density than either glucose or methanol. growth at high glycerol concentrations up 12% did not cause any significant repression growth. culture, 83 g l(-1) dry weight production 550 mg l(-1), obtained less 32 h cultivation fed-batch fermentation employing intermittent feeding glycerol. promoter-based system showed specific rate required much simpler process MOX system, demonstrating that can be practical alternative promoter large-scale H.