467 Gut–brain mechanisms of COVID-19 in wild type mice

• DOI: 10.1017/cts.2024.1060
• Publication Date: 2025-03-26T03:58:20Z
• Authors: Grant Talkington; Ismael Saifudeen; Gregory Jaye Bix
• Keywords: 2019-20 coronavirus outbreak

Objectives/Goals: SARS-CoV-2 infection has been shown to impact multiple organ systems, including the brain, and is associated with increased cognitive decline in vulnerable populations. The gut microbiome may play a significant role modulating these effects, as shifts microbiota composition have linked inflammation systemic disease processes. Methods/Study Population: To explore interactions, we conducted an acute COVID-19 study using 12-week-old C57 mice intranasally inoculated 1x10^4 PFU of Mouse Adapted 10 (MA-10) strain SARS-CoV-2. On day 5 postinfection, performed 16S ribosomal RNA sequencing on fecal samples, analyzed QIIME2 DeSeq2, assess alterations identify changes metabolic pathways inflammatory responses. Results/Anticipated Results: Analysis revealed notable during phase infection, related immune regulation. These suggest that early disruptions contribute host response COVID-19. Discussion/Significance Impact: Our findings indicate leads rapid microbiome, which could underlie impacting progression. insights highlight potential for microbiome-targeted therapies mitigate morbidity warrant further investigation.