Taurine supplementation: involvement of cholinergic/phospholipase C and protein kinase A pathways in potentiation of insulin secretion and Ca2+handling in mouse pancreatic islets

• DOI: 10.1017/s0007114510001820
• Publication Date: 2010-07-01T09:01:24Z
• Authors: Rosane A. Ribeiro; Emerielle C. Vanzela; Camila A. M. Oliv...; Maria L. Bonfleur; Antonio C. Boschero; Everardo M. Carneiro
• Keywords: Pancreatic Islets; IBMX

Taurine (TAU) supplementation increases insulin secretion in response to high glucose concentrations rodent islets. This effect is probably due an increase Ca 2+ handling by the islet cells. Here, we investigated possible involvement of cholinergic/phospholipase C (PLC) and protein kinase (PK) A pathways this process. Adult mice were fed with 2 % TAU drinking water for 30 d. The killed pancreatic islets isolated collagenase method. Islets from TAU-supplemented showed higher presence 8·3 m -glucose, 100 μ -carbachol (Cch) 1 -3-isobutyl-1-methyl-xanthine (IBMX), respectively. Cch was accompanied a intracellular mobilisation PLC β2 expression. uptake but similar when challenged plus IBMX. also expression PKAα protein. may play role cation accumulation, since amount these significantly reduced PKA inhibitors: N -[2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide (H89) PK inhibitor-(6–22)-amide (PKI). In conclusion, glucose, favouring both influx internal , effects seem involve activation PLC–inositol-1,4,5-trisphosphate cAMP–PKA pathways.