Differential expression of liver proteins between obesity-prone and obesity-resistant rats in response to a high-fat diet

• DOI: 10.1017/s0007114511000651
• Publication Date: 2011-05-03T09:16:51Z
• Authors: Xia Wang; Jung-Won Choi; Jeong In Joo; Dong Hyun Kim; Tae Seok Oh; Duk Kwon Choi; Jong Won Yun
• Keywords: Lipogenesis; Proteome

Rodents respond to a chronic high-fat diet (HFD) in two ways: some readily become obese (obesity prone, OP) and others do not resistant, OR). Although several hypotheses have been proposed, the mechanisms underlying inter-individual susceptibility diet-induced obesity remain be fully defined. In present study, two-dimensional gel electrophoresis (2-DE) combined with matrix-assisted laser desorption ionisation time-of-flight MS was carried out for identification of differentially expressed liver proteins OP OR rats fed HFD, an attempt discover marker involved and/or resistance rat liver. The 2-DE analysis demonstrated that forty spots from 380 visualised were regulated between groups. Among these spots, twelve rats, reaching statistical significance. Of these, five already linked obesity; however, seven or identified first time study. order validate proteomic results gain insight into metabolic changes groups, we further confirmed expression pattern interest by Western blot analysis. Combined revealed reduced lipogenesis increased fat oxidation achieved livers rats. conclusion, study is important advance over previous steps required should prove valuable search pathogenesis humans.