Screening the ability of natural feed ingredients to interfere with the adherence of enterotoxigenicEscherichia coli(ETEC) K88 to the porcine intestinal mucus

• DOI: 10.1017/s0007114513003024
• Publication Date: 2013-09-19T10:51:35Z
• Authors: Gemma González-Ortiz; José Francisco Pérez; Rafael Gustavo He...; Francesc Molist; Rufino Jiménez-Díaz; Susana María Mart...
• Keywords: Enterotoxigenic Escherichia coli

The inhibition of the attachment bacteria to intestine by receptor analogues could be a novel approach prevent enterotoxigenic Escherichia coli (ETEC) K88-induced diarrhoea in piglets. objective present study was screen ability different feed ingredients (FI) bind ETEC K88 (adhesion test, AT) and block its porcine intestinal mucus (blocking BT) using vitro microtitration-based models. In AT, wheat bran (WB), casein glycomacropeptide (CGMP) exopolysaccharides exhibited highest adhesion ( P < 0·001). BT, WB, CGMP locust bean (LB) reduced number attached For WB LB, fractionation based on their carbohydrate components subsequently carried out, each fraction evaluated individually. None fractions as did original extract, suggesting that protein or glycoprotein involved recognition process. With regard LB fractions, water-extractable material 0·001) similar extract 0·001), indicating, this case, galactomannans phenolic compounds responsible for conclusion, among FI screened, soluble extracts obtained from anti-adhesive properties against BT. These results suggest they may good candidates included diets weaned piglets prevention diarrhoea.