Beneficial effects of soluble dietary Jerusalem artichoke (Helianthus tuberosus) in the prevention of the onset of type 2 diabetes and non-alcoholic fatty liver disease in high-fructose diet-fed rats

• DOI: 10.1017/s0007114514001421
• Publication Date: 2014-06-26T13:00:19Z
• Authors: Wan-Ching Chang; Huijuan Jia; Wanping Aw; Kenji Saito; Sumio Hasegawa; Hisanori Kato
• Keywords: Nutrigenomics

Jerusalem artichoke (JA) has the potential to attenuate lipid disturbances and insulin resistance (IR), but underlying mechanisms are not well understood. In present study, we elucidated physiological responses of JA intervention with a comprehensive transcriptome analysis. Wistar rats were fed control diet, 60 % fructose-enriched diet (FRU), or FRU 10 ( n 6–7) for 4 weeks. An oral glucose tolerance test was carried out on day 21. Liver samples collected biochemical global gene expression analyses (GeneChip ® Rat Genome 230 2.0 Array, Affymetrix). Fructose feeding resulted in IR hepatic TAG accumulation; dietary supplementation significantly improved these changes. Transcriptomic profiling revealed that malic enzyme 1 Me1 ), associated fatty acid synthesis; decorin Dcn related fibrosis; cytochrome P450, family 1, subfamily a, polypeptide 2 Cyp1a2 ) nicotinamide phosphoribosyltransferase Nampt inflammation, differentially altered by FRU, whereas genes. We established first time molecular driving beneficial effects prevention type diabetes non-alcoholic liver disease. propose may be onset diseases.