Development and evaluation of a colloidal gold immunochromatographic assay based on recombinant protein CatL1D for serodiagnosis of sheep fasciolosis

• DOI: 10.1017/s0022149x19000919
• Publication Date: 2019-11-04T09:55:27Z
• Authors: W. Xifeng; Q. Mengfan; Z. Kai; Z. Guowu; L. Jing; W. Lixia; Q. Jun; M. Qingling; G. Shasha; H. Yunfu; C. Xuepeng
• Keywords: Fasciolosis; Gold standard (test); Hepatica

Abstract Fasciolosis is a zoonotic parasitic disease that seriously endangers the development of animal husbandry and human health. In order to develop rapid serological diagnostic method for fasciolosis in ruminants, CatL1D CatB4 genes Fasciola hepatica were amplified by reverse transcription polymerase chain reaction (PCR) cloned, respectively, then CatL-B fusion gene (MeCatL-B) was constructed splicing overlap extension PCR technique. The recombinant rCatL1D, rCatB4 rMeCatL-B proteins prepared prokaryotic expression, protein with high specificity sensitivity screened via indirect enzyme-linked immunosorbent assay. Using selected rCatL1D as antigen, we developed colloidal gold immunochromatographic assay (CGIA) detecting F. -specific antibodies, 426 serum samples slaughtered sheep used evaluate CGIA results showed (100%, 96.67%) higher than those (94.29%, 80%) (91.43%, 90%). Compared standard post-mortem inspection, 100% 97%, consistency rate between these two methods 99.3%. These confirmed based on could be promising approach diagnosis because its specificity.