Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

Male Proteomics 0301 basic medicine Plasmodium Plasmodium berghei proteome Protozoan Proteins mosquito Mycology & Parasitology 03 medical and health sciences LIVER STAGES ookinete surface SEXUAL DEVELOPMENT Animals micronemes Life Cycle Stages Science & Technology SURFACE PROTEIN 0707 Veterinary Sciences PARASITOLOGY GAMETE DEVELOPMENT MASS-SPECTROMETRY microgamete LIFE-CYCLE PROTEIN DISULFIDE-ISOMERASE Insect Vectors Malaria 3. Good health Culicidae 570 Life sciences; biology Parasitology Female MALARIA PARASITE Life Sciences & Biomedicine SUBCELLULAR-LOCALIZATION AEDES-AEGYPTI Research Article
DOI: 10.1017/s0031182012000133 Publication Date: 2012-02-16T11:42:32Z
ABSTRACT
SUMMARYHere we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.
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