Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls

• DOI: 10.1017/s0031182012000133
• Publication Date: 2012-02-16T11:42:32Z
• Authors: M. N. WASS; R. STANWAY; A. M. BLAGBOROUGH; K. LAL; J. H. PRIETO; D. RAINE; M. J. E. STERNBERG; A. M. TALMAN; F. TOMLEY; J. YATES; R. E. SINDEN
• Keywords: Proteome; Plasmodium berghei; Plasmodium (life cycle); Cell function; Model Organism

SUMMARY Here we discuss proteomic analyses of whole cell preparations the mosquito stages malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) Plasmodium berghei. We also include critiques proteomes two fractions from purified namely micronemes surface. Whereas summarise key biological interpretations data, try to identify methodological constraints have met, only some which were able resolve. Recognising need translate potential current genome sequencing into functional understanding, report our efforts develop more powerful combinations methods for in silico prediction protein function location. applied this analysis proteome male gamete, a whose very simple structural organisation facilitated interpretation data. Some predictions made now been supported by ongoing tagging genetic knockout studies. hope discussion may assist future