Proteomic analysis of Plasmodium in the mosquito: progress and pitfalls
Male
Proteomics
0301 basic medicine
Plasmodium
Plasmodium berghei
proteome
Protozoan Proteins
mosquito
Mycology & Parasitology
03 medical and health sciences
LIVER STAGES
ookinete
surface
SEXUAL DEVELOPMENT
Animals
micronemes
Life Cycle Stages
Science & Technology
SURFACE PROTEIN
0707 Veterinary Sciences
PARASITOLOGY
GAMETE DEVELOPMENT
MASS-SPECTROMETRY
microgamete
LIFE-CYCLE
PROTEIN DISULFIDE-ISOMERASE
Insect Vectors
Malaria
3. Good health
Culicidae
570 Life sciences; biology
Parasitology
Female
MALARIA PARASITE
Life Sciences & Biomedicine
SUBCELLULAR-LOCALIZATION
AEDES-AEGYPTI
Research Article
DOI:
10.1017/s0031182012000133
Publication Date:
2012-02-16T11:42:32Z
AUTHORS (11)
ABSTRACT
SUMMARYHere we discuss proteomic analyses of whole cell preparations of the mosquito stages of malaria parasite development (i.e. gametocytes, microgamete, ookinete, oocyst and sporozoite) of Plasmodium berghei. We also include critiques of the proteomes of two cell fractions from the purified ookinete, namely the micronemes and cell surface. Whereas we summarise key biological interpretations of the data, we also try to identify key methodological constraints we have met, only some of which we were able to resolve. Recognising the need to translate the potential of current genome sequencing into functional understanding, we report our efforts to develop more powerful combinations of methods for the in silico prediction of protein function and location. We have applied this analysis to the proteome of the male gamete, a cell whose very simple structural organisation facilitated interpretation of data. Some of the in silico predictions made have now been supported by ongoing protein tagging and genetic knockout studies. We hope this discussion may assist future studies.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (85)
CITATIONS (33)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....