Genotype replacement of the human parainfluenza virus type 2 in Croatia between 2011 and 2017 – the role of neutralising antibodies

• DOI: 10.1017/s0950268818001693
• Publication Date: 2018-06-18T07:07:40Z
• Authors: M. Šantak; M. Lang Balija; G. Mlinarić Galin...; S. Ljubin Sternak; T. Vilibić-Čavlek; I. Tabain
• Keywords: Likelihood Functions; 0303 health sciences; HN Protein; genotype ; fusion protein ; hemagglutinin-neuraminidase ; human parainfluenza virus type 2 ; neutralising antibodies; Adolescent; Genotype; Croatia; Guinea Pigs; Infant; Middle Aged; Antibodies, Viral; Antibodies, Neutralizing; Parainfluenza Virus 2, Human; 3. Good health; 03 medical and health sciences; Age Distribution; Child, Preschool; Chlorocebus aethiops; Animals; Epitopes, B-Lymphocyte; Humans; Female; Child; Phylogeny

Abstract Previously we reported on the HPIV2 genotype distribution in Croatia 2011–2014. Here expand this period up to 2017 and confirm that G1a has replaced G3 from Our hypothesis was G1a-to-G3 replacement is an antibody-driven event. A cross-neutralisation with anti-HPIV2 sera specific for either or revealed presence of genotype-specific antigenic determinants. By profound, silico analyses three potential B cell epitopic regions were identified hemagglutinin neuraminidase (regions 314–361 474–490) fusion protein (region 440–484). The region does not show any unique site between isolates, five differentially glycosylated sites isolates neuraminidase. All positively selected codons found be located 314–316 474–490 what indicates a strong positive selection reveals these are susceptible evolutionary pressure possibly caused by antibodies gives verification our neutralising key determinant inherently complex adaptive evolution region.