RFRP-3 synchronized with photoperiods regulates the seasonal reproduction of striped hamsters

• DOI: 10.1017/s0967199421000496
• Publication Date: 2021-12-01T13:34:43Z
• Authors: Huiliang Xue; Jinhui Xu; Lei Chen; Lei Zhao; Ming Wu; Laixiang Xu
• Keywords: Male; 0301 basic medicine; Photoperiod; Reproduction; Neuropeptides; Luteinizing Hormone; Gonadotropin-Releasing Hormone; 03 medical and health sciences; Cricetinae; Animals; Female; RNA, Messenger; Seasons; Follicle Stimulating Hormone; Melatonin

The purpose of this study was to investigate the effect RFRP-3 synchronized with photoperiods on regulating seasonal reproduction striped hamsters. hamsters were raised separately under long-day (LD; 16 h light/8 dark), medium-day (MD; 12 light/12 dark) or short-day (SD; 8 light/16 conditions for weeks. and gonadotropin-releasing hormone (GnRH) mRNA levels in hypothalamus, testis ovaries three groups detected using reverse transcription polymerase chain reaction (RT-PCR). Melatonin (MLT), follicle-stimulating (FSH) luteinizing (LH) concentrations serum enzyme-linked immunosorbent assay (ELISA). correlation between GnRH FSH LH also analyzed. MLT negatively regulated expression RFRP-3. Significant differences existed groups, which positively correlated concentrations. hypothalamus significantly higher than those testis. lower female male SD conditions, while testes LD conditions. decreased RFRP neuron activity,