Metabolism of halauxifen acid is regulated by genes located on wheat chromosome 5A

• DOI: 10.1017/wsc.2024.24
• Publication Date: 2024-04-15T09:46:21Z
• Authors: Olivia A. Landau; Jeanaflor Crystal...; Dean E. Riechers
• Keywords: Aegilops

Abstract Allohexaploid wheat ( Triticum aestivum L.) is tolerant to halauxifen-methyl (HM) via rapid detoxification of the phytotoxic form HM, halauxifen acid (HA), non-phytotoxic metabolites. Previous research utilizing ‘Chinese Spring’ (CS) wheat, alien substitution (i.e., endogenous chromosome pair substituted with a homoeologous from diploid Sears’ goatgrass Aegilops searsii M. Feldman & Kislev) (AS), or nullisomic-tetrasomic (NT) lines indicated plants lacking 5A are more sensitive than CS HM. We hypothesized increased HM sensitivity these results losing gene(s) on associated HA metabolism, which leads reduced rate relative CS. To compare abundance among AS, CS, substitution, and NT during time course, two excised-leaf studies using unlabeled liquid chromatography–mass spectrometry analyses were performed. accumulated did, each line at 8, 12, 24 h after treatment (HAT). Furthermore, only displayed greater (2.4- 3.8-fold, depending point). In contrast, abundances in possessing not different all points. When compared nullisomic 5D-tetrasomic (N5D-T5A) similar abundance, whereas 5A-tetrasomic 5D (N5A-T5D) approximately 3-fold 12 HAT. These biochemically support hypothesis that genes encoding HA-detoxifying enzyme(s) located corroborate findings previous greenhouse phenotypic experiments. Future experimentation needed identify characterize enzymes involved detoxification, may include cytochrome P450 monooxygenases, unknown oxidases, UDP-dependent glucosyltransferases, or, potentially, transcription factors regulate expression detoxification.