Bifunctional Trehalose Anode Incorporating Two Covalently Linked Enzymes Acting in Series

• DOI: 10.1021/ac2014417
• Publication Date: 2011-08-03T21:22:14Z
• Authors: Michelle Rasmussen; Richard West; Jim Burgess; Irene Lee; Daniel Scherson
• Keywords: Phosphofructokinase 2

A bienzymatic electrode incorporating trehalase (Tre) and glucose oxidase (GOx) covalently bound to the surface of Pt through a functionalized thiol linker (Tre|GOx|Pt) has been designed assembled its catalytic properties examined by chemical electrochemical methods in aqueous phosphate buffer solutions (PBS, pH 7.4). Exposure Tre|GOx|Pt PBS containing trehalose (Tr) subsequent polarization at 0.6 V versus Ag/AgCl yielded after few minutes well-defined steady-state currents ascribed oxidation hydrogen peroxide generated GOx-mediated (Gl) produced Tre-mediated dissociation Tr. Plots [Tr] over range examined, i.e., 5–25 mM, were found be linear. Implications these results toward development an implantable biofuel cell as autonomous energy conversion device for insects are discussed.