Utility of Ion-Mobility Spectrometry for Deducing Branching of Multiply Charged Glycans and Glycopeptides in a High-Throughput Positive ion LC-FLR-IMS-MS Workflow

• DOI: 10.1021/acs.analchem.0c01954
• Publication Date: 2020-11-09T18:43:54Z
• Authors: Edward G. Pallister; Matthew S. F. Choo; Ian Walsh; Jien Nee Tai; Shi Jie Tay; Yuan Sheng Yang; Say Kong Ng; Pauline M. Rudd; Sabine L. Flitsch; Terry Nguyen-Khuong
• Keywords: Glycomics; Ion-mobility spectrometry; Branching (polymer chemistry)

High-throughput glycan analysis has become an important part of biopharmaceutical production and quality control. However, it is still a significant challenge in the field glycomics to easily deduce isomeric structures, especially high-throughput manner. Ion mobility spectrometry (IMS) excellent tool for differentiating structures. demonstrations utility IMS workflows such as liquid chromatography-fluorescence-mass (LC-FLR-MS) have been limited with only small amount collision cross section (CCS) data available. In particular, fragments obtained positive ion mode are comparison those negative despite being widely used glycomics. Here, we describe TWCCSN2 from LC-FLR-IMS-MS workflow mode. We selection RapiFluor-MS (RFMS) labeled N-glycans also glycopeptides. how able distinguish glycopeptides using both intact fragment-based sequencing experiments mode, without significantly altering nature analysis. For first time, were successfully use determine branching RFMS glycans. Further, highlight that glycans was similar Finally, show approach can shared structural features nonisomeric workflow.