Smartphone-Based Electrochemical Biosensors for Directly Detecting Serum-Derived Exosomes and Monitoring Their Secretion

• DOI: 10.1021/acs.analchem.1c04910
• Publication Date: 2022-01-27T20:57:46Z
• Authors: Jing Su; Shixing Chen; Yanzhi Dou; Zhihan Zhao; Xiaolong Jia; Xianting Ding; Shiping Song
• Keywords: CD63; Diagnostic biomarker

Exosomes are potential biomarkers, which play an important role in early diagnosis and prognosis prediction of cancer-related diseases. Nevertheless, direct quantification exosomes biological fluid, especially point-of-care tests (POCTs), remains extremely challenging. Herein, we developed a sensitive portable electrochemical biosensor combination with smartphones for quantitative analysis exosomes. The improved double-antibody sandwich method-based poly-enzyme signal amplification was adopted to detect We could as low 7.23 ng CD63-positive 5 μL serum within 2 h. Importantly, demonstrated that the worked well microliter-level cell culture supernatant. holds great detection CD-63-expressing prostate disease because were less detected from patient serum. Also, used monitor secretion drug therapy, showing close relationship between concentration cisplatin. biosensing platform provides novel way toward POCT other diseases via biomarker expression levels