Structural Analysis Provides Mechanistic Insight into Nicotine Oxidoreductase from Pseudomonas putida

• DOI: 10.1021/acs.biochem.6b00963
• Publication Date: 2016-11-14T18:03:14Z
• Authors: Margarita A. Tara...; Kim D. Janda; Karen N. Allen
• Keywords: Pseudomonas putida

The first structure of nicotine oxidoreductase (NicA2) was determined by X-ray crystallography. Pseudomonas putida has evolved nicotine-degrading activity to provide a source carbon and nitrogen. establishes NicA2 as member the monoamine oxidase family. Residues 1–50 are disordered may play role in localization. nicotine-binding site proximal isoalloxazine ring flavin shows an unusual composition classical aromatic cage (W427 N462). active architecture is consistent with proposed binding deprotonated form substrate flavin-dependent oxidation pyrrolidone C–N bond followed nonenzymatic hydrolysis.