Redox Kinetics of the Amyloid-β-Cu Complex and Its Biological Implications

• DOI: 10.1021/acs.biochem.8b00133
• Publication Date: 2018-09-28T22:00:04Z
• Authors: Paul Girvan; Xiangyu Teng; Nicholas J. Brooks; Geoffrey S. Baldwin; Liming Ying
• Keywords: Amyloid (mycology)

The ability of the amyloid-β peptide to bind redox active metals and act as a source radical damage in Alzheimer's disease has been largely accepted contributing disease's pathogenesis. However, kinetic understanding molecular mechanism, which underpins this generation, yet be reported. Here we use sensitive fluorescence approach, reports on oxidation state metal bound can therefore shed light kinetics. We confirm that goes via low populated, reactive intermediate reaction proceeds Component I coordination environment rather than II. also show while reduction step readily occurs (on 10 ms time scale) it is rate-limiting for cycling.