Integrative Analysis of lncRNA–mRNA Coexpression in Human Lung Epithelial Cells Exposed to Dimethyl Selenide-Derived Secondary Organic Aerosols

• DOI: 10.1021/acs.chemrestox.0c00516
• Publication Date: 2021-03-03T20:50:00Z
• Authors: C. M. Sabbir Ahmed; Biplab Chandra Paul; Yumeng Cui; Alexander L. Frie; Abigail Burr; Rohan Kamath; Jin Y. Chen; Tara M. Nordgren; Roya Bahreini; Ying-Hsuan Lin
• Keywords: Aerosols; 03 medical and health sciences; 0302 clinical medicine; Organoselenium Compounds; Humans; Epithelial Cells; RNA, Long Noncoding; RNA, Messenger; RNA-Seq; Lung; Cells, Cultured

Dimethyl selenide (DMSe) is one of the major volatile organoselenium compounds released into atmosphere through plant metabolism and microbial methylation. DMSe has been recently revealed as a precursor secondary organic aerosol (SOA), its resultant SOA possesses strong oxidizing capability toward thiol groups that can perturb several biological pathways in human airway epithelial cells linked to genotoxicity, DNA damage, p53-mediated stress responses. Mounting evidence suggested long noncoding RNAs (lncRNAs) are involved responses internal environmental stimuli. However, underlying molecular interactions remain be elucidated. In this study, we performed integrative analyses lncRNA–mRNA coexpression transformed bronchial BEAS-2B cell line exposed DMSe-derived SOA. We identified total 971 differentially expressed lncRNAs derived from O3 OH oxidation DMSe. Gene ontology (GO) network analysis cis-targeted genes showed significant enrichment apoptosis, response pathways. trans-Acting lncRNAs, including PINCR, PICART1, DLGAP1-AS2, LINC01629, known associated with carcinogenesis, also altered expression treated DMSe-SOA. Overall, study highlights regulatory role gene induced by DMSe-SOA exposure.