Development of an Integrated Platform to Assess the Physicochemical and Toxicological Properties of Wood Combustion Particulate Matter

• DOI: 10.1021/acs.chemrestox.2c00183
• Publication Date: 2022-09-06T15:37:58Z
• Authors: Dilpreet Singh; Dereje Damte Tassew; Jordan Nelson; Marie-Cecile G. C...; Ilias G. Kavouras; Philip Demokritou; Yohannes Tesfaigzi
• Keywords: Combustion products

Wood burning contributes to indoor and ambient particulate matter (PM) pollution has been associated with increased morbidity mortality. Here, we present an integrated methodology that allows generate, sample, characterize wood smoke derived from different moisture contents representative combustion conditions using pine as a model. Flaming, smoldering, incomplete were assessed for low-moisture pine, whereas both high-moisture investigated under flaming conditions. Real-time monitoring of carbon monoxide, volatile organic compounds, aerosol number concentration/size in was performed. The PM size-fractionated, sampled, characterized elemental/organic carbon, functional groups, inorganic elements. Bioactivity by measuring the sterile alpha motif (SAM) pointed domain containing ETS (E-twenty-six) transcription factor (SPDEF) gene promoter activity human embryonic kidney 293 (HEK-293T) cells, biomarker mucin expression. Findings showed content condition significantly affected elemental composition PM0.1 well its bioactivity. Also, given scenario, chemistry bioactivity differed considerably size. Importantly, contained highest abundance oxygenated saturated aliphatic group [H–C–O] also biologically most potent stimulating SPDEF activity, suggesting role compounds such carbohydrates sugar alcohols (that contain [H–C–O]) driving mucus-related respiratory outcomes. Our platform enables further well-controlled parametric studies combination vitro vivo approaches link parameters acute chronic inhalation health effects smoke.