Strong Inhibitory Effect of Heme on hIAPP Fibrillation

• DOI: 10.1021/acs.chemrestox.7b00170
• Publication Date: 2017-08-08T22:04:06Z
• Authors: Jinming Wu; Jie Zhao; Zhen Yang; Hailing Li; Zhonghong Gao
• Keywords: 0301 basic medicine; 03 medical and health sciences; Spectrometry, Fluorescence; Microscopy, Electron, Transmission; Circular Dichroism; Humans; Protoporphyrins; Heme; Electrophoresis, Gel, Pulsed-Field; Islet Amyloid Polypeptide; Protein Binding

The deposition of human islet amyloid polypeptide (hIAPP) within β-cells is implicated in the etiology of type 2 diabetes mellitus (T2Dm). It was reported that heme could bind to hIAPP. We speculate that binding may affect the aggregation of hIAPP. In this study, UV-vis spectroscopy was used to detect the interaction pattern between the heme and hIAPP. ThT and Bis-ANS fluorescence assay, circular dichroism spectroscopy, gel electrophoresis assay, and transmission electron microscopy were employed to study the effect of heme on the aggregation of hIAPP. We found that heme dramatically inhibited hIAPP aggregation, even partially dismantled hIAPP aggregates by preventing its conformational changes. Moreover, a similar inhibitory effect was also observed on mutant hIAPP. In the compared group, the inhibitory effects of protoporphyrin on hIAPP and its mutants aggregation were weaker. Similarly, its effect on the dismantlement of the aggregates was also weaker. On the basis of these results, we revealed that the heme iron center was not required for the inhibitory effect on hIAPP but affected the binding affinity of heme to hIAPP. Besides Arg11 and His18, other hydrophobic residues of hIAPP may also play important roles in heme binding. Our results may help to develop an in-depth understanding of the interaction between heme and hIAPP, which would be helpful in designing new therapeutic strategies against T2Dm.