Monoclonal Antibodies for the Detection of a Specific Cyclic DNA Adduct Derived from ω-6 Polyunsaturated Fatty Acids

• DOI: 10.1021/acs.chemrestox.8b00111
• Publication Date: 2018-07-12T09:37:47Z
• Authors: Marcin Dyba; Brandon da Silva; Heidi Coia; Yanqi Hou; Sumire Noguchi; Jishen Pan; Deborah Berry; Karen Creswell; Jacek Krzeminski; Dhimant Desai; Shantu Amin; David Yang; Fung-Lung Chung
• Keywords: 0301 basic medicine; Aldehydes; Adenosine; Antibodies, Monoclonal; Enzyme-Linked Immunosorbent Assay; Cell Separation; DNA; Hep G2 Cells; Flow Cytometry; 3. Good health; DNA Adducts; Mice; 03 medical and health sciences; Tandem Mass Spectrometry; Fatty Acids, Omega-6; Carcinogens; Hepatocytes; Animals; Epoxy Compounds; Humans; Chromatography, Liquid

Lipid peroxidation of polyunsaturated fatty acids (PUFAs) is an endogenous source α,β-unsaturated aldehydes that react with DNA producing a variety cyclic adducts. The mutagenic adducts, specifically those derived from oxidation ω-6 PUFAs, may contribute to the cancer promoting activities associated PUFAs. ( E)-4-Hydroxy-2-nonenal (HNE) unique product PUFAs oxidation. HNE reacts deoxyguanosine (dG) yielding 1, N2-propanodeoxyguanosine adducts (HNE-dG). Earlier studies showed can also be oxidized its epoxide (EH), and EH deoxyadenosine (dA) forming well-studied εdA substituted etheno Using liquid chromatography-based tandem mass spectroscopic (LC-MS/MS) method, we previously reported detection EH-derived 7-(1',2'-dihydroxyheptyl)-1, N6-ethenodeoxyadenosine (DHHεdA) as novel background adduct in rodent human tissues. formation, repair, mutagenicity DHHεdA biological consequences cells have not been investigated. To understand roles carcinogenesis, it important develop immuno-based assay detect In this study describe development monoclonal antibodies against application cells.