Phosphorus Deficiency Promoted Hydrolysis of Organophosphate Esters in Plants: Mechanisms and Transformation Pathways

• DOI: 10.1021/acs.est.1c02396
• Publication Date: 2021-07-12T08:39:02Z
• Authors: Qing Liu; Xiaolei Wang; Jian Zhou; Xiaoyong Yu; Menglin Liu; Yao Li; Hongwen Sun; Lingyan Zhu
• Keywords: 0301 basic medicine; 2. Zero hunger; China; 03 medical and health sciences; Hydrolysis; Esters; Phosphorus; Organophosphates; Environmental Monitoring; Flame Retardants

The biotransformation of organophosphate esters (OPEs) in white lupin (Lupinus albus) and wheat (Triticum aestivum L.) was investigated hydroponic experiments with different phosphorus (P)-containing conditions. hydrolysis rates OPEs followed the order triphenyl phosphate (TPHP) > tri-n-butyl (TnBP) tris(1,3-dichloro-2-propyl) (TDCPP). Hydrolysis accelerated at P-deficient conditions, faster took place than wheat. Coincidingly, production acid phosphatase (ACP) both plants promoted, much higher intracellular extracellular ACPs were observed under In vitro revealed that ACP a key enzyme to hydrolyze OPEs. significantly correlated Hirshfeld charges, calculated by density functional theory, oxygen atom single P-O bond. Using ultra-high-performance liquid chromatography coupled Orbitrap Fusion mass spectrometer, 30 metabolites successfully identified. Some these metabolites, such as sulfate-conjugated products, hydration cysteine-conjugated products TPHP, reductively dechlorinated TDCPP, for first time plants. It is noteworthy may transform into many hydroxylated special attention should be paid their potential environmental effects.