Strategy for Mitigating Antibiotic Resistance by Biochar and Hyperaccumulators in Cadmium and Oxytetracycline Co-contaminated Soil

• DOI: 10.1021/acs.est.1c03434
• Publication Date: 2021-10-25T23:43:45Z
• Authors: Yuhao Fu; Mingyun Jia; Fang Wang; Ziquan Wang; Zhi Mei; Yongrong Bian; Xin Jiang; Marko Virta; James M. Tiedje
• Keywords: 11832 Microbiology and virology; 2. Zero hunger; Drug Resistance, Microbial; Oxytetracycline; 15. Life on land; 01 natural sciences; Anti-Bacterial Agents; 3. Good health; Environmental sciences; Manure; Soil; Biodegradation, Environmental; Genes, Bacterial; 13. Climate action; Charcoal; Humans; Other agricultural sciences; Soil Microbiology; Cadmium; 0105 earth and related environmental sciences

The global prevalence of antibiotic resistance genes (ARGs) is of increasing concern as a serious threat to ecological security and human health. Irrigation with sewage and farmland application of manure or biosolids in agricultural practices introduce substantial selective agents such as antibiotics and toxic metals, aggravating the transfer of ARGs from the soil environment to humans via the food chain. To address this issue, a hyperaccumulator (Sedum plumbizincicola) combined with biochar amendment was first used to investigate the mitigation of the prevalence of ARGs in cadmium and oxytetracycline co-contaminated soil by conducting a pot experiment. The addition of biochar affected the distribution of ARGs in soil and plants differently by enhancing their prevalence in the soil but restraining transmission from the soil to S. plumbizincicola. The planting of S. plumbizincicola resulted in an increase in ARGs in the soil environment. A structural equation model illustrated that mobile genetic elements played a dominant role in shaping the profile of ARGs. Taken together, these findings provide a practical understanding for mitigating the prevalence of ARGs in this soil system with complex contamination and can have profound significance for agricultural management in regard to ARG dissemination control.