Assessment and Comparison of Early Developmental Toxicity of Six Per- and Polyfluoroalkyl Substances with Human Embryonic Stem Cell Models

• DOI: 10.1021/acs.est.3c10758
• Publication Date: 2024-04-30T19:47:48Z
• Authors: Miaomiao Zhao; Nuoya Yin; Renjun Yang; Shichang Li; Shuxian Zhang; Francesco Faiola
• Keywords: Perfluorooctanoic acid; Developmental toxicity; Ciliogenesis; Embryoid body

Per- and polyfluoroalkyl substances (PFAS) are extensively utilized in varieties of products tend to accumulate the human body including umbilical cord blood embryos/fetuses. In this study, we conducted an assessment comparison potential early developmental toxicity perfluorooctanoic acid (PFOA), undecafluorohexanoic (PFHxA), heptafluorobutyric acid, perfluorooctanesulfonate (PFOS), perfluorohexanesulfonate, perfluorobutyric at noncytotoxic concentrations relevant exposure using models based on embryonic stem cells both three-dimensional embryoid (EB) monolayer differentiation configurations. All six compounds influenced determination cell fate by disrupting expression associated markers and, some instances, even led alterations formation cystic EBs. The cilia-related gene IFT122 was significantly inhibited. Additionally, PFOS PFOA inhibited ciliogenesis, while specifically reduced cilia length. Transcriptome analysis revealed that altered 1054 genes disrupted crucial signaling pathways such as WNT TGF-β, which play integral roles transduction critical for development. These results provide precise comprehensive insights into adverse health effects these PFAS directly concerning