MAPK-CncC Signaling Pathways Regulate the Antitoxic Response to Avermectin-Induced Oxidative Stress in Juvenile Chinese Mitten Crab, Eriocheir sinensis

• DOI: 10.1021/acs.est.4c11404
• Publication Date: 2025-01-30T21:39:41Z
• Authors: Yi Huang; Yuhang Hong; Shu Wu; Yan Sun; Qiang Huang; Xiaodan Hu; Wei Long; Zhiqiu Huang
• Keywords: Chinese mitten crab; Avermectin

This study delves into the adverse effects of AVM, emphasizing oxidative stress induction in Chinese mitten crab, Erocheir sinensis, and role MAPK-CncC signaling pathway mediating antioxidative response. Our findings reveal a dose-dependent impairment growth performance, alongside occurrence stress. The activity CAT superoxide dismutase increased significantly all treatments (0.2, 2, 20 μg/L) while lipid peroxidation level rose median (2 high (20 concentration groups. T-AOC levels decreased reactive oxygen species significantly. Transcriptomic insights indicated activation pathway, critical upregulating genes associated with detoxification defense. Knocking down CncC, homologue vertebrate Nrf2 gene, by dsRNA E. sinensis resulted down-regulation reduced survival tolerance to AVM exposure. By using an vitro cell culture system, we observed similar downregulations when hepatopancreas cells were treated ML385, CncC specific inhibitor. Moreover, rapid phosphorylation JNK protein was upon When inhibited, more vulnerable AVM-induced robust expression gene blocked.