Antibiotic Resistome and Its Association with Bacterial Communities during Sewage Sludge Composting

• DOI: 10.1021/acs.est.5b01012
• Publication Date: 2015-05-27T15:44:19Z
• Authors: Jian-Qiang Su; Bei Wei; Wei-Ying Ou-Yang; Fu-Yi Huang; Yi Zhao; Hui-Juan Xu; Yong-Guan Zhu
• Keywords: Resistome; Sewage sludge; Soil microbiology

Composting is widely used for recycling of urban sewage sludge to improve soil properties, which represents a potential pathway spreading antibiotic resistant bacteria and genes soils. However, the dynamics resistance (ARGs) underlying mechanisms during composting were not fully explored. Here, we high-throughput quantitative PCR 16S rRNA gene based illumina sequencing investigate ARGs bacterial communities lab-scale in-vessel sludge. A total 156 unique mobile genetic elements (MGEs) detected encoding almost all major classes antibiotics. with significantly increased abundance diversity, distinct patterns, enriched composting. Marked shifts in community structures compositions observed composting, Actinobacteria being dominant phylum at late phase The large proportion may partially explain increase patterns correlated structures, suggesting that dynamic was strongly affected by phylogenetic These results imply direct application compost on field lead spread abundant