Changes in Neurotransmitter Profiles during Early Zebrafish (Danio rerio) Development and after Pesticide Exposure

• DOI: 10.1021/acs.est.5b05665
• Publication Date: 2016-02-11T22:34:28Z
• Authors: Sara Tufi; Pim Leonards; Marja Lamoree; Jacob de Boer; Juliette Legler; Jessica Legradi
• Keywords: Danio; Lymnaea stagnalis; Neurotransmitter Agents

During early development, neurotransmitters are important stimulants for the development of central nervous system. Although different neuronal cell types during zebrafish (Danio rerio) is well-studied, little known levels neurotransmitters, their precursors and metabolites how these affected by exposure to environmental contaminants. A method based on hydrophilic interaction liquid chromatography coupled tandem mass spectrometry has been applied first time embryos larvae study five neurotransmitter systems in parallel, including dopaminergic–andrenergic, glutaminergic–GABAnergic, serotoninergic, histaminergic, cholinergic systems. Our enables quantification whole from period zygote free-swimming 6 days postfertilization (dpf). We observed a developmental stage-dependent pattern with clear differences between 2 following days. Whereas steadily increased, showed peak at 3 dpf. After several pesticides, significant concentrations were observed. revealed new insights about usefulness our approach neurotoxicity studies.