Aerobic Degradation of Sulfadiazine by Arthrobacter spp.: Kinetics, Pathways, and Genomic Characterization

• DOI: 10.1021/acs.est.6b02231
• Publication Date: 2016-07-30T15:27:52Z
• Authors: Yu Deng; Yanping Mao; Bing Li; Chao Yang; Tong Zhang
• Keywords: Arthrobacter

Two aerobic sulfadiazine (SDZ) degrading bacterial strains, D2 and D4, affiliated with the genus Arthrobacter, were isolated from SDZ-enriched activated sludge. The degradation of SDZ by two isolates followed first-order decay kinetics. half-life time complete was 11.3 h for strain 46.4 D4. Degradation kinetic changed nongrowth to growth-linked when glucose introduced as cosubstrate, accelerated biodegradation rate observed after adaption period. Both could degrade into 12 products via 3 parallel pathways, which 2-amino-4-hydroxypyrimidine detected principal intermediate product toward pyrimidine ring cleavage. Compared five Arthrobacter strains reported previously, D4 only sole carbon source. draft genomes same completeness 99.7%, compared other related species. Overall, these shared high genomic similarities s-triazine-degrading sp. AK-YN10 sulfonamide-degrading bacteria Microbacterium C448. In addition, contained a few significant regions difference may carry functional genes involved in sulfonamide degradation.