Biotoxin Tropolone Contamination Associated with Nationwide Occurrence of Pathogen Burkholderia plantarii in Agricultural Environments in China

• DOI: 10.1021/acs.est.7b05915
• Publication Date: 2018-03-28T12:01:12Z
• Authors: Xiaoyu Liu; Xiaoyan Fan; Haruna Matsumoto; Yanxia Nie; Zhimin Sha; Kunpeng Yi; Jiuyue Pan; Yuan Qian; Mengchao Cao; Yihu Wang; Guonian Zhu; Mengcen Wang
• Keywords: Tropolone

Tropolone, a biotoxin produced by the agricultural pathogen Burkholderia plantarii, exerts cytotoxicity toward wide array of biota. However, due to lack quantitative and qualitative approach, both B. plantarii occurrence tropolone contamination in environments remain poorly understood. Here, we presented sensitive reliable method for detection artificial, plant, environmental matrices tropolone-targeted gas chromatography–triple-quadrupole tandem mass spectrometry analysis. Limits were 10 colony-forming units (CFU)/mL 0.017 μg/kg, respectively. In series simulation trials, found that from 108 CFU/mL between 0.006 107.8 mg/kg cell-population-dependent manner, regardless habitat. Correlation analysis clarified reflection density level with R2 values 0.9201 0.9756 (p < 0.01). Through nationwide pilot study conducted China, was observed at 0.014–0.157 paddy soil rice grains, subsequent redundancy revealed organic matter be dominant factor, having positive correlation contamination. this context, our results imply potential ecological dietary risks posed long-term exposure trace levels are concern.