Method Development for Effect-Directed Analysis of Endocrine Disrupting Compounds in Human Amniotic Fluid

• DOI: 10.1021/acs.est.9b04255
• Publication Date: 2019-10-04T15:12:45Z
• Authors: Hanna M. Dusza; Elwin Janssen; Rakesh Kanda; Juliette Legler
• Keywords: Solid phase extraction; Xenobiotic; Nonylphenol

The developing fetus represents a highly sensitive period of exposure to endocrine disrupting compounds (EDCs). However, risk assessment EDCs is hampered by the lack data on direct in utero exposure. In this study, we developed robust analytical methodology for identification wide range known and unknown full-term amniotic fluid (AF). First, method extraction fractionation broad polar nonpolar was validated. Maximal recoveries reference minimal interference from matrix were achieved with combination solid phase dispersive liquid/liquid extraction. Bioassay analysis using cell-based reporter gene assays revealed estrogenic, androgenic, dioxin-like activity AF extract corresponding 1.4 nmol EEQ/L, 76.6 pmol DHT-EQ/L, 10.1 TEQ/L, respectively. Targeted 13 xenobiotics, phytoestrogens, endogenous hormones that partly contributed bioassay activity. Separation complex mixture chemicals reversed-phase chromatographic subsequent fractions over polarity, indicating diverse (unidentified) substances potential ED here first methodological step an effect-directed approach identify biologically active fetal environment.