6PPD-Quinone: Revised Toxicity Assessment and Quantification with a Commercial Standard

• DOI: 10.1021/acs.estlett.1c00910
• Publication Date: 2022-01-11T19:54:04Z
• Authors: Zhenyu Tian; Melissa Gonzalez; Craig A. Rideout; Haoqi Nina Zhao; Ximin Hu; Jill Wetzel; Emma Mudrock; C. Andrew James; Jenifer K. McIntyre; Edward P. Kolodziej
• Keywords: Toxicant; Isotope dilution

Stormwater exposure can cause acute mortality of coho salmon (Oncorhynchus kisutch), and 6PPD-quinone (6PPD-Q) was identified as the primary causal toxicant. Commercial standards 6PPD-Q recently became available; their analysis highlighted a systematic high bias in prior reporting concerning 6PPD-Q. A commercial standard used to re-confirm toxicity estimates juvenile develop liquid chromatography-tandem mass spectrometry analytical method for quantification. Peak area responses were ∼15 times higher than those in-house standards, updated LC50 value (95 ng/L) ∼8.3-fold lower that previously reported. These data support relative comparisons occurrence while confirming substantial lethality While environmental concentrations are expected be lower, also more toxic calculated should categorized “very highly toxic” pollutant aquatic organisms. Isotope dilution-tandem methods enabled accurate quantification (limits <10 within samples.