Evolutionary Engineering Improved d-Glucose/Xylose Cofermentation of Yarrowia lipolytica

• DOI: 10.1021/acs.iecr.0c00896
• Publication Date: 2020-06-01T17:22:59Z
• Authors: Linlin Zhou; Zhiqiang Wen; Zedi Wang; Yuwei Zhang; Rodrigo Ledesma-A...; Mingjie Jin
• Keywords: Yarrowia; Snf3; Pentose; Xylose metabolism; Metabolic Engineering

The recombinant strain Y. lipolytica XYL+ can utilize xylose to produce microbial lipids efficiently. However, its uptake is severely delayed in the presence of d-glucose. In this study, adaptive laboratory evolution (ALE) strains medium containing and d-glucose analogue 2-deoxyglucose (dG) performed. After four stages over a total 64 days, for first time, we obtained an (yl-XYL+*04*10) that cometabolize glucose, with diminished glucose repression. Xylose kinetics showed it could efficiently 10 g/L dG or Transcriptional profiling analysis revealed relative expression level xylose-specific transporter genes (encoded by YALI0_C04730g YALI0_D00363g) was significantly upregulated. addition, missense mutations N373T G270A YALI0_E23287g (encoding transporter) YALI0_E15488g hexokinase) respectively, were found. It implied these are important gene targets responsible improved utilization evolved lipolytica. Our work provides new approach breeding paved way future pentose metabolic engineering.