Recognition of HClO in Live Cells with Separate Signals Using a Ratiometric Fluorescent Sensor with Fast Response

• DOI: 10.1021/acs.iecr.5b01904
• Publication Date: 2015-08-26T15:58:55Z
• Authors: Jiangli Fan; Huiying Mu; Hao Zhu; Jianjun Du; Na Jiang; Jingyun Wang; Xiaojun Peng
• Keywords: Hypochlorous acid; Parts-per notation; BODIPY; Pyrrole

Hypochlorous acid (HClO), one of the reactive oxygen species (ROS), is a key microbicidal agent which used for natural defense; however, it also involved in several human diseases. Although efforts have been made to develop HClO-selective fluorescent sensors, most them display delayed response time and ratiometric sensors are rare. In current work, we 3-pyrrole BODIPY dye as new sensor (BRClO) HClO. BRClO exhibited 177-fold fluorescence ratio (F505 nm/F585 nm) enhancement presence HClO over other ROS with fast (completed within 1 s). Confocal imaging showed separated signals exogenous live cells. addition, could be easily prepared test strips detect aqueous solution tap water.