Formation Dynamics of Cell-Loading Alginate Droplets in the Microtube Dripping and Cryo-Cross-Linking Process for Cell-Entrapped Cryogel Beads as the Biocatalysts toward Phenyllactic Acid Biosynthesis

• DOI: 10.1021/acs.iecr.8b00831
• Publication Date: 2018-05-11T18:22:17Z
• Authors: Wei Zhang; Yujun Yang; Tingting Guan; Jintao Guan; Sanlong Zheng; Bingbing Chen; Junxian Yun
• Keywords: Bioconversion; Oil droplet

The dynamic formation of alginate droplets containing Lactobacillus buchneri cells by the microtube dripping method was investigated experimentally. Supermacroporous cell-entrapped cryogel beads were then fabricated via cryo-cross-linking these cell-loaded under freezing conditions and used as biocatalysts for synthesis phenyllactic acid bioconversion. evolution interface, velocity, microthread length, droplet diameter during process studied high-speed imaging together with computational fluid dynamics modeling. results showed that detachment controlled interaction gravity, surface tension, viscous drag forces. With increase size from 0.5 to 2.2 mm, length microthread, velocity mean increased 5.9, 3.7, 1.7 times, respectively. There is a good agreement between simulation experimental regarding performance droplets. prepared methods have narrow distributions, macroporous structures, high porosity 88.1%. These had remarkable biocatalysis ability conversion substrate phenylpyruvic acid. Compared free cells, biotransformation ratio about 95.9% after recycled two indicating could be employed new class production thus potential application in bioconversion processes.