Dichlororesorufin-Based Colorimetric and Fluorescent Probe for Ultrasensitive Detection of Mercury Ions in Living Cells and Zebrafish

• DOI: 10.1021/acs.iecr.8b04386
• Publication Date: 2018-12-10T20:49:06Z
• Authors: Qingxia Duan; Xiaoyu Lv; Caiyun Liu; Zhuofan Geng; Fenfen Zhang; Wenlong Sheng; Zuokai Wang; Pan Jia; Zilu Li; Hanchuang Zhu; Baocun Zhu
• Keywords: 01 natural sciences; 0104 chemical sciences

In this work, a simple dichlororesorufin-based colorimetric and fluorescent probe R-Hg with the recognition receptor of carbonothioate was designed, synthesized, and exploited to determine mercury ions in aqueous solution and living cells as well as in zebrafish. After a series of analytical tests, probe R-Hg showed some excellent characteristics, such as ultrasensitivity, high selectivity, and good water solubility. The probe solution exhibited a very large fluorescence enhancement (about 150-fold) upon addition of Hg2+. Additionally, probe R-Hg exhibited excellent sensitivity, which could quantificationally detect Hg2+ at the nanomolar level, and the detection limit was 0.49 nM. Moreover, probe R-Hg could be used as a “naked-eye” probe for monitoring Hg2+, because the color of its solution transformed remarkably from yellow into pink with the participation of Hg2+. Importantly, probe R-Hg has potential application value and has been successfully applied to the determination of Hg2+ in practical water sa...