Evaluation of the Quality of a High-Resistant Starch and Low-Glutelin Rice (Oryza sativa L.) Generated through CRISPR/Cas9-Mediated Targeted Mutagenesis

• DOI: 10.1021/acs.jafc.0c02995
• Publication Date: 2020-08-10T20:43:46Z
• Authors: Dongshu Guo; Xitie Ling; Xiaogeng Zhou; Xiao Li; Jinyan Wang; Shi Qiu; Yuwen Yang; Baolong Zhang
• Keywords: Glutelin; Resistant Starch; Brown rice

A high-resistant starch (RS) and low-glutelin diet is beneficial for the health of patients with diabetes kidney diseases. Rice an important food crop worldwide. Previous studies have demonstrated that downregulating expression rice branching enzyme IIb (SBEIIb) affected composition structure starch. However, there has been no report about generating loss-of-function mutants SBEIIb using cultivars as recipients. In this study, we adopted a CRISPR/Cas9 system to induce site-specific mutations at locus in elite japonica cultivar derived from Low Glutelin Content-1 (LGC-1) successfully obtained two independent transgene-free sbeIIb/Lgc1 mutant lines. lines, apparent amylose content (AAC) was increased by approximately 1.8-fold RS reached 6%. The glutelin 2%, maintaining trait recipient cultivar. formation mechanism explored analyzing fine structures properties According X-ray diffraction pattern lipid content, high lines attributed amylose–lipid complex. Further analyses nutritional quality revealed soluble sugar contents, especially sucrose unsaturated fatty acids, significantly. This research expected facilitate cultivation application functional suitable