Metabolic Engineering and Adaptive Evolution of Clostridium beijerinckii To Increase Solvent Production from Corn Stover Hydrolysate

• DOI: 10.1021/acs.jafc.0c03048
• Publication Date: 2020-07-02T14:17:55Z
• Authors: Jinle Liu; Yu Jiang; Jun Chen; Junjie Yang; Weihong Jiang; Wei Zhuang; Hanjie Ying; Sheng Yang
• Keywords: Clostridium beijerinckii; Corn stover; Corncob; Lignocellulosic Biomass; Pentose

The production of acetone–butanol–ethanol by solventogenic Clostridium using lignocellulosic biomass can be a potential alternative to petroleum-based butanol. However, previous studies on nondetoxified lignocellulose hydrolysate could not provide better results when compared those in synthetic medium. In this study, we engineered the pentose pathway beijerinckii NCIMB 8052, which was then subjected adaptive laboratory evolution gradient mixture medium and pretreated corn stover enzymatic (CSH) prepared according National Renewable Energy Laboratory (NREL) standard. final resultant strain CIBTS1274A produced 20.7 g/L total solvents NREL CSH diluted 6% initial sugars, supplemented with ammonium acetate. This performance comparable that corn-based addition, successfully used scale-up operation corncob at Lignicell Refining Biotechnologies Ltd., once only commercial biobutanol industry world.