Circ09863 Regulates Unsaturated Fatty Acid Metabolism by Adsorbing miR-27a-3p in Bovine Mammary Epithelial Cells

• DOI: 10.1021/acs.jafc.0c03917
• Publication Date: 2020-07-21T08:48:23Z
• Authors: Zhi Chen; Jingpeng Zhou; Mengjie Wang; Jiahua Liu; Longfei Zhang; Juan J. Loor; Yusheng Liang; Hua Wu; Zhangping Yang
• Keywords: Fatty acid synthesis; Fatty Acid Metabolism; Bovine milk

Fatty acid composition plays a key role in regulating flavor and quality of milk. Therefore, order to improve milk quality, it is particularly important investigate regulatory mechanisms fatty metabolism. Circular RNAs (circRNAs) regulate expression genes associated with several biological processes including In this study, high-throughput sequencing was used detect differentially expressed bovine mammary tissue at early lactation peak lactation. Circ09863 profiles were influenced by the stage. Functional studies epithelial cells (BMECs) revealed that circ09863 promotes triglyceride (TAG) synthesis together increased content unsaturated acids (C16:1 C18:1). These results suggested partly responsible for modulating Additionally, software prediction identified miR-27a-3p binding site sequence. Overexpression BMECs led decreased TAG synthesis. However, overexpression (pcDNA-circ09863) significantly reduced enhanced gene synthase (FASN), target miR-27a-3p. Overall, data suggest relieves inhibitory effect on FASN subsequently composition. Together, these provide new research avenues theoretical bases quality.