Antifungal Mechanism of Volatile Organic Compounds Produced by Bacillus subtilis CF-3 on Colletotrichum gloeosporioides Assessed Using Omics Technology

• DOI: 10.1021/acs.jafc.1c00640
• Publication Date: 2021-04-26T12:24:13Z
• Authors: Ke Wang; Zhen Qin; Shiyuan Wu; Pengyu Zhao; Chaoying Zhen; Haiyan Gao
• Keywords: Ergosterol; Colletotrichum

Bacillus subtilis is commonly used as a biocontrol bacterium owing to its strong antifungal activity, broad-spectrum inhibition, and general safety. In this study, the inhibitory effects of volatile organic compounds (VOCs) produced by B. CF-3 on Colletotrichum gloeosporioides, major destructive phytopathogen litchi anthracnose, were analyzed using proteomics transcriptomics. Differentially expressed genes (DEGs) proteins (DEPs) indicated that inhibition C. gloeosporioides VOCs downregulated expression related cell membrane fluidity, wall integrity, energy metabolism, production wall-degrading enzymes. Particularly, those important DEGs DEPs ergosterol biosynthetic biosynthesis unsaturated fatty acids are most significantly influenced. 2,4-di-tert-butylphenol, characteristic component VOCs, also showed similar effect gloeosporioides. Our results provide theoretical basis for potential application in postharvest protection fruits vegetables.