Spatially Resolved Metabolomics and Lipidomics Reveal Salinity and Drought-Tolerant Mechanisms of Cottonseeds

• DOI: 10.1021/acs.jafc.1c01598
• Publication Date: 2021-07-13T02:49:56Z
• Authors: Bingbing Liu; Xiaoxiao Wang; Kun Li; Zongwei Cai
• Keywords: 2. Zero hunger; 0301 basic medicine; Salinity; Cottonseed Oil; 15. Life on land; 6. Clean water; Droughts; Plant Breeding; 03 medical and health sciences; Stress, Physiological; 13. Climate action; Lipidomics; Metabolomics

In the current era of global climate change, environmental stresses, especially drought and salt, have impaired the growth and productivity of crops, e.g., cotton. Understanding the mechanisms of plants' adaptation to these abiotic stresses is crucial to breed stress-tolerant crop species. In the present study, integrated metabolomics, lipidomics, and mass spectrometry imaging (MSI) were used to discover the spatial distribution of differential metabolites and lipids in two cottonseed cultivars with contrasting drought and salt tolerance properties. Seventeen differential metabolites and 125 differential lipids were identified. Their possible roles in augmenting stress tolerance were illustrated, which were involved in reactive oxygen species scavenging, osmotic adjustment, and cell membrane structure reconstruction. MSI analysis provided a visualization of nine differential lipids and four differential metabolites in cottonseeds with varied abundances and distributions. The results may help understand cottonseeds' convictive metabolic and lipidomic regulatory networks in coping with salinity and drought stresses and give new insights into the stress-tolerance traits relevant to other crops.