Sodium Alginate Modulates Immunity, Intestinal Mucosal Barrier Function, and Gut Microbiota in Cyclophosphamide-Induced Immunosuppressed BALB/c Mice

• DOI: 10.1021/acs.jafc.1c02294
• Publication Date: 2021-06-21T14:54:01Z
• Authors: Juan Huang; Jinli Huang; Yao Li; Yilu Wang; Fahe Wang; Xia Qiu; Xvliang Liu; Huajun Li
• Keywords: Lachnospiraceae; Intestinal mucosa; Intestinal Permeability

This study investigated the protective effects of sodium alginate (SA) on gut microbiota, immunity, and intestinal mucosal barrier function in cyclophosphamide-induced immunosuppressed BALB/c mice. SA alleviated spleen tissue damage restored impaired immune functions, such as increasing organ index, decreasing splenic T lymphocytes, markedly secretion serum immunoglobulins cytokines In addition, reversed injury increased permeability by upregulating expression tight junction proteins. Moreover, decreased inflammation reducing d-lactic acid (D-LA) lipopolysaccharide (LPS) concentrations downregulating toll-like receptor 4 (Tlr4) mitogen-activated protein kinase (Mapk) pathway expression. Furthermore, significantly abundance beneficial bacteria (Lactobacillus, Roseburia, Lachnospiraceae NK4A136) pathogenic (Helicobacter, Peptococcus, Tyzzerella) intestine determined 16S rRNA gene high-throughput sequencing. conclusion, our provides a scientific basis for functional food modulating microbiota protecting against indicates that has potential application enhancing immunity.