tae-miR399-UBC24 Module Enhances Freezing Tolerance in Winter Wheat via a CBF Signaling Pathway

• DOI: 10.1021/acs.jafc.1c04316
• Publication Date: 2021-11-03T10:12:40Z
• Authors: Kankan Peng; Yu Tian; Xianze Sun; Chunhua Song; Zhipeng Ren; Yuzhuo Bao; Jinpu Xing; Yuanshan Li; Qinghua Xu; Jing Yu; Da Zhang; Jing Cang
• Keywords: Freezing tolerance; Cold stress

Although the regulation of Pi homeostasis by miR399 has been studied in various plant species, its underlying molecular mechanism response to freezing stress is still poorly understood. In this work, we found that expression tae-miR399 and target gene TaUBC24 tillering nodes strong cold-resistant winter wheat cultivar Dongnongdongmai1 (Dn1) was not only significantly altered after severe winters but also responsive short-term stress. physically interacted with TaICE1. Enhanced tolerance observed for tae-miR399-overexpressing Arabidopsis lines. Under stress, overexpression ultimately decreased AtUBC24, inhibiting degradation AtICE1, which increased genes involved CBF signaling pathway starch metabolism promoted activities antioxidant enzymes. These results will improve our understanding through miR399-UBC24 module plays a cardinal role regulating mediation downstream pathways.