Lingonberry Anthocyanins Inhibit Hepatic Stellate Cell Activation and Liver Fibrosis via TGFβ/Smad/ERK Signaling Pathway

• DOI: 10.1021/acs.jafc.1c05384
• Publication Date: 2021-11-04T17:14:37Z
• Authors: Guokun Zhang; Yunyao Jiang; Xin Liu; Yongyan Deng; Bin Wei; Liyan Shi
• Keywords: Hepatic stellate cell; Hydroxyproline; Hepatic fibrosis

Phytochemicals from lingonberry have rich pharmacological value and may play an essential role in treating liver diseases. We investigated the regulatory of anthocyanins (LA) on HSC activation vitro fibrogenesis vivo. The viability HSCs treated with LA was significantly reduced a dose-dependent manner at concentration 25-100 μg/mL, which monomers also proliferation via IC50 assay. inducer transforming growth factor β1 (TGFβ1) effector α-smooth muscle actin (α-SMA) were all decreased both protein RNA levels by LA. Moreover, alleviated CCl4-induced fibrosis rats, reducing collagen aggregation production decreasing hydroxyproline (HYP) malondialdehyde (MDA) tissue. indexes serum reversed index function rats. Furthermore, antioxidant enzymes, including superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), tissue increased upon treatment Importantly, promoted hepatic parenchymal cell inhibited expression TGFβ/Smad/extracellular regulated kinase (ERK) signaling pathway-related genes. This study demonstrates anti-liver activity investigates its mechanism, provide novel strategy for using lingonberry.