5-Hydroxymethylfurfural Exerts Negative Effects on Gastric Mucosal Epithelial Cells by Inducing Oxidative Stress, Apoptosis, and Tight Junction Disruption

• DOI: 10.1021/acs.jafc.2c00269
• Publication Date: 2022-03-21T14:37:29Z
• Authors: Yanting Qiu; Xiaorong Lin; Zhongzheng Chen; Bin Li; Yuanyuan Zhang
• Keywords: Occludin

5-Hydroxymethylfurfural (5-HMF) is a processing byproduct present in foods that are consumed daily by humans, and the diet principal route for human exposure to it. However, its adverse effects on gastric epithelial cells not fully understood. Based half inhibitory concentration value, concentrations of HMF 2, 4, 8, 16 mM were selected this study. After 5-HMF treatment 24 h, number living decreased 89.61 ± 0.48, 77.30 0.57, 58.75 0.36, 19.61 0.40% control, respectively. Apoptosis activated through both death receptor mitochondrial pathways was confirmed be primary mode HMF-induced cell death. Further analysis revealed reactive oxygen species (ROS) levels GES-1 increased 1.7-6.5 fold after 5-HMF. Moreover, inhibition ROS N-acetylcysteine blocked apoptosis proliferation suppression, indicating oxidative stress important apoptosis. Besides, treatment, gene expressions occludin ZO-1 reduced 1.1-3.4 2.0-9.4 fold, The surface morphology tight junction-related protein expression also destructive effect junction integrity. Our research highlights potential mechanism toxicity provides additional information health risks epithelium.