Verification of Bound Aminoguanidine as the Marker Residue for the Banned Antibiotic, Nitrovin, in Pig Tissues

• DOI: 10.1021/acs.jafc.2c00843
• Publication Date: 2022-06-06T20:04:39Z
• Authors: Gemma Regan; Philip Mullen; Mary Moloney; Karl De Ruyck; Terence Fodey; Sarah E. Millar; Steven Crooks; Anna Gadaj; Chris Elliott; Martin Danaher
• Keywords: 570; Nitrofurans; Swine; Nitrovin; General Chemistry; 530; Guanidines; 01 natural sciences; Drug Residues; Anti-Bacterial Agents; 0104 chemical sciences; 3. Good health; Liver; Animals; General Agricultural and Biological Sciences

Nitrovin (NTV) belongs to a class of antibiotics called nitrofurans, which are classified as nonallowed pharmacologically active substances that do not have a maximum residue limit listed in EU legislation. The objectives of this study were to confirm aminoguanidine (AGN) as a suitable marker residue to monitor NTV abuse and to investigate its persistence in porcine tissues. In this work, pigs were fed with NTV-medicated feed (50 mg/kg), and tissues (kidney, muscle, and liver) and plasma were collected on different withdrawal days. All samples were analyzed for bound AGN, total AGN, and the parent drug NTV itself. The highest concentrations of AGN residues were found in the liver, while the lowest were in muscle. Parent NTV was only detected in the kidney at low levels on day 0 of withdrawal. The findings are in support of using AGN as the marker residue for monitoring the illegal use of NTV in animal-derived products.