Nuciferine Ameliorates Nonesterified Fatty Acid-Induced Bovine Mammary Epithelial Cell Lipid Accumulation, Apoptosis, and Impaired Migration via Activating LKB1/AMPK Signaling Pathway

• DOI: 10.1021/acs.jafc.2c06133
• Publication Date: 2022-12-27T12:37:41Z
• Authors: Jinxia Li; Chenchen Zhao; Menglin Liu; Linfang Chen; Yiwei Zhu; Wenwen Gao; Xiliang Du; Yuxiang Song; Xinwei Li; Guowen Liu; Lin Lei; Haihua Feng
• Keywords: Lipotoxicity; NEFA

High blood concentrations of nonesterified fatty acids (NEFAs) provoke various metabolic disorders and are associated with mammary tissue injury decreased milk production in dairy cows. Nuciferine, an alkaloid found Nelumbo nucifera leaves, has great potential for correcting lipid metabolism derangements lipotoxicity. In this study, we evaluated the lipotoxicity induced by excessive NEFA bovine epithelial cells (bMECs) investigated whether nuciferine alleviates NEFA-induced underlying molecular mechanisms. We that (1.2 2.4 mM) accumulation, apoptosis, migration ability impairment bMECs, whereas could ameliorate these disarrangements, as indicated decreasing triglyceride content, protein abundance SREBP-1c, cytoplasmic cytochrome c, cleaved caspase-3 increasing PPARα ability. Moreover, reverse LKB1/AMPK signaling inhibition, protective effect on caused was abrogated AMPK inhibitor dorsomorphin. Furthermore, transfection LKB1 siRNA (si-LKB1) largely abolished activation AMPK. Overall, can protect bMECs from impaired activating pathway.