2-Hydrazinoterephthalic Acid as a Novel Negative-Ion Matrix-Assisted Laser Desorption/Ionization Matrix for Qualitative and Quantitative Matrix-Assisted Laser Desorption/Ionization–Mass Spectrometry Analysis of N-Glycans in Peach Allergy Research

• DOI: 10.1021/acs.jafc.2c06822
• Publication Date: 2022-12-21T12:25:46Z
• Authors: Huiwen Wang; Yuexia Gao; Quan He; Jiancong Liao; Shiwen Zhou; Yaqin Liu; Cheng Guo; Xian Li; Xiaoyong Zhao; Yuanjiang Pan
• Keywords: Prunus persica; Ions; 0301 basic medicine; 03 medical and health sciences; Polysaccharides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Lasers; Food Hypersensitivity

Glycans recently attracted considerable attention as the proposal of cross-reactive carbohydrate determinants for food allergy. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) is powerful in analyzing biomolecules, while its applications in glycans are still challenging. Herein, a novel reactive matrix-assisted laser desorption/ionization (MALDI) matrix, 2-hydrazinoterephthalic acid, was rationally designed and synthesized. It provides uniform co-crystallization with glycans and only produces deprotonated ions with high intensities in the negative-ion mode. In combination with sinapic acid, a rapid and high-throughput method was established for on-target analysis of glycans with a superior limit of detection at the femtomole level and a good linearity (R2 > 0.999). Furthermore, the established method was successfully applied to quantify N-glycans in different cultivars and tissues of peach [Prunus persica (L.) Batsch]. Our work suggests the potential role of N-glycans as biomarkers for food-borne allergy and lays a methodological foundation for the elucidation of the possible relationship between carbohydrate epitopes and food allergy.