Comprehensive Screening for EPA/DHA-Structured Phospholipids in Aquatic Products by a Specific Precursor Ion Scanning-Based HILIC-MS/MS Method

• DOI: 10.1021/acs.jafc.3c00505
• Publication Date: 2023-05-11T10:45:35Z
• Authors: Jing Xue; Lijun Ge; Honghai Wang; Jingjing Liang; Qingcheng Wang; Weibo Lu; Yiwei Cui; Hujun Xie; Shikai Jian; Danping Jin; Qizhi Jin; Ting Li; Qing Shen
• Keywords: Phosphatidylethanolamine

Comprehensive screening for functional substances from natural resources is always a hot research topic. Eicosapentaenoic acid- (EPA) and docosahexaenoic acid (DHA)-structured phospholipids (PLEPA/DHA) have versatile cardiovascular benefits as well superior bioavailability. Herein, the abundance of PLEPA/DHA in 16 aquatic products was specifically selectively screened using recently developed precursor ion scan-driven hydrophilic interaction chromatography–mass spectrometry (PreIS-HILIC/MS) method with fatty acyl moieties EPA (m/z 301.6) DHA 327.6) locked. The aim focused on characteristics differences varieties contents EPA/DHA-structured phosphatidylcholine (PCEPA/DHA) phosphatidylethanolamine (PEEPA/DHA) molecular species. A total 80 molecules were identified these sources, including 47 PCEPA/DHA 33 PEEPA/DHA. After analysis, PC 16:0/20:5 16:0/22:6 are present all at high levels. Antarctic krill found to be best resource (2574.69 μg·g–1), followed by mackerel (2330.11 salmon (2109.91 Farrer's scallop (1883.59 while abalone contained lowest level (310.44 μg·g–1). Besides, sea cucumber bass highest EPA-structured DHA-structured ether phospholipids, respectively, which could highly recommended dietary sources special phospholipids. Finally, multiple discrepancies between revealed multivariate statistical analysis. These findings improve awareness composition content products, providing reference their integrated development.