Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals the Mechanism of Guvermectin Promoting Seed Germination in Direct-Seeded Rice under Chilling Stress

• DOI: 10.1021/acs.jafc.3c00559
• Publication Date: 2023-05-02T13:29:01Z
• Authors: Chongxi Liu; Chenxu Li; Hui Bing; Junwei Zhao; Lei Li; Peng Sun; Tingting Li; Dandan Du; Junlei Zhao; Xiangjing Wang; Wensheng Xiang
• Keywords: Panicle

Rice direct seeding technology has been considered as a promising alternative to traditional transplanting because of its advantages in saving labor and water. However, the poor emergence seedling growth caused by chill stress are main bottlenecks wide-scale adoption direct-seeded rice Heilongjiang Province, China. Here, we found that natural plant regulator guvermectin (GV) effectively improved seed germination under chilling stress. Results from 2 year field trials showed seed-soaking with GV not only enhanced rate but also increased panicle number per grain panicle, resulting 9.0 6.8% increase yield rice, respectively. Integrative physiological, transcriptomic, metabolomic assays revealed promoted mainly enhancing activities α-amylase antioxidant enzymes (superoxide dismutase, peroxidase, catalase), increasing contents soluble sugar protein, improving biosynthesis glutathione flavonoids, well activating gibberellin-responsive transcription factors inhibiting abscisic acid signaling pathway. These findings indicate good potential improve establishment even