Natural Sweetener, Glycyrrhetinic Acid 3-O-Mono-beta-d-glucuronide, for Postprandial Hyperglycemia Management

• DOI: 10.1021/acs.jafc.3c07188
• Publication Date: 2024-02-09T18:18:15Z
• Authors: Lichun Guo; Mengqing Zhang; Ying Fei; Wei Zhao
• Keywords: Nutraceutical

This study examines the inhibitory effects of a range sweeteners on α-glucosidase. Our findings revealed that only one natural sweetener, namely, glycyrrhetinic acid 3-O-mono-beta-d-glucuronide (GAMG), derived from licorice, exhibited mixed-type inhibition against α-glucosidase with IC50 value 0.73 ± 0.05 mg/mL. The fluorescence intensity was quenched by GAMG in formation an α-glucosidase–GAMG complex. has been shown to induce conformational changes α-glucosidase, likely through hydrogen bonding, van der Waals force, and alkyl–alkyl interactions amino residues, including Arg 281, Leu 283, Trp 376, Asp 404, 443, 481, 518, Phe 525, Ala 555, 616. Additional animal validation experiments demonstrated slowed starch digestion, thereby attenuating postprandial glycemic response. Taken together, these provide evidence is sweetener potent activity selectively targets supports use as which holds high biological may be beneficial for managing hyperglycemia.